services and equipment
OMICS LABORATORY SERVICES
01. Sample Preparation
1.1. Tissue Homogenization
1.2. Nucleic acid and Protein Extraction
1.3. Amplicon Purification
1.4. Next-generation sequencing Library Preparation
02. Spectrophotometry
2.1. Nucleic acid Concentration and Purity
2.2. Total Protein Quantification
2.3. Enzyme-linked Immunosorbent Assay (ELISA)
2.4. Reducing Sugar Analysis (Dinitrosalicylic acid Test)
2.5. Total Phenolic Content Analysis (Folin-Ciocalteu Method)
2.6. Radical Scavenging Activity Assay (DPPH)
03. Fluorometry
3.1. Total DNA/RNA/Protein Quantification
04. Polymerase Chain Reaction
4.1. Gene detection using End-point PCR
4.2. Transcript/Gene detection using Quantitative real-time PCR
05. Electrophoresis
5.1. Agarose gel electrophoresis (AGE)
5.2. 1D gel electrophoresis (SDS-PAGE)
5.3. 1D gel electrophoresis staining (Coomasie blue)
5.4. Western blotting
5.5. 2D gel electrophoresis
06. Flow Cytometry
6.1. Direct FACS (flourophore-conjugated primary antibody, e.g. RPE, FITC, Alexa Fluor)c
FACILITY
Laboratory Facility
Routine nucleic acid workflow
includes: autoclave, universal centrifuge, refrigerated centrifuge, thermal cycler, water bath, gel documentation system, ice maker, spectrophotometer, biofreezer (-80°C), and freezer (-20°C)
2. Café-type Workstation
BIOINFORMATICS SERVICE
1. Next Generation Sequencing Workflow
2. Capillary Sequencing Workflow
3. Phylogenetic Analysis
4. Remote Server Usage
EQUIPMENT
1. Laboratory Equipment
1.1. Spectrophotometer
1.2. Flow Cytometer
1.3. Quantitative Real-time PCR
1.4. Fluorometer
1.5. Gradient End-point PCR
1.6. Tissue Homogenizer
1.7. Tissue Sonicator
1.8. Refrigerated centrifuge
1.9. Centrifuge, Microfuge
2. MinION Sequencer
3. Sample Storage
3.1. -20°C Freezer
3.2. -80°C Freeze
4. Bioinformatics Server Rental
4.1. Data Access with Storage (DAWS) e
4.2. Data Access without Storage (DAWOS)
- Basic Molecular Biology
- Molecular Biology Camp
- Real-Time PCR Diagnostic
- Nanopore Sequence
- Molecular Biology and Diagnostic
- Next Generation Sequencing Workflow
- Capillary Sequencing Workflow
- eDNA Metabarcoding Workflow
- Intro to Linux Command Line/BASH scripting
- GWAS Analysis
- Phylogenetic Analysis
STATE OF THE ART EQUIPMENT
The advanced equipment of PGC Mindanao are housed temporarily in the different laboratories of CSM: Biotechnology, Molecular Biology and Extraction Laboratory.
PGC MINDANAO EQUIPMENT
With the recent establishment of Philippine Genome Center Mindanao through a seed funding worth P40 million equipment from the Department of Science and Technology-Philippine Council for Health Research and Development (DOST-PCHRD), omics research is now within closer reach to the Mindanao community and intends to be a catalyst for growth and development in the Island.
Watch PGC Mindanao's
HIGH-THROUGHPUT EQUIPMENT
STATE-OF-THE-ART SEQUENCING EQUIPMENT
